Facilities

NMR Facility

The departmental facility is equipped with 500 and 600 MHz NMR instruments (Varian INOVA). The director of the NMR facility is Dr. Lazaros Kakalis.

Varian 500 NMR Spectrometer

The Varian Inova 500 spectrometer uses a 51 mm bore, 28-shim, 11.7 T superconducting Oxford magnet. The console consists of three r.f. channels, each with waveform generation and pulse shaping capabilities, a Z pulsed field gradient unit, and a variable temperature unit. The available probes are a 5mm dual broadband (Ag109-P31, H1/F19) probe with z-gradient, a 5mm H1/C13/N15 triple resonance probe with Z gradient, and a 10mm broadband (N15-P31, H1) probe.

Varian 600 NMR Spectrometer

The Varian Inova 600 spectrometer uses a 51 mm bore, 40-shim, actively-shielded, 14.1 T superconducting Oxford magnet. The console consists of three r.f. channels, each with waveform generation and pulse shaping capabilities, a deuterium decoupling module, a XYZ pulsed field gradient unit, and a variable temperature unit. The available probes are a 5mm H1/C13/N15 triple resonance probe with XYZ gradients and a 5mm dual broadband (N15-P31, H1/F19) probe with z-gradient.

 

FTMS Facility

The departmental facility is equipped with an Apex-ultra 70 hybrid fourier transform mass spectrometer (Bruker Daltonics). The FTMS facility is managed by Dr. Roman Brukh.

The FTMS is equipped with a 7.0 T actively shielded, high-stability magnet, and an Apollo II Dual ESI/MALDI source to provides simultaneous operation in both modes. The ESI source has an ion funnel and a spray chamber with a grounded off-axis sprayer. The MALDI source has computer controlled X-Y stage for scout-385 MTP target plates. Additional instrument capabilities are HPLC and nanospray experiments. Fragmentation methods include in-source collision activated dissociation (IS-CAD), collision cell activated dissociation (C-CAD), sustained off-resonance irradiation collision activated dissociation (SORI-CAD), infrared multi-photon dissociation (IRMPD), and electron capture dissociation (ECD).

Sample applications are compound elucidation, drug impurity analysis, natural product characterization, high-end proteomics studies, and metabolomics research.